Performing restriction digests consists of the following process:
- Adding enzyme to DNA to slice the gene sequence at a specific point
- Timing the procedure
- Washing off the reagent
- Analyzing the end result
Process/Skill Questions:
- How is the specific point of the gene sequence determined for a certain trait?
- Why does the laboratory technician need to use an enzyme to slice a small portion of the DNA?
- What are the steps involved in the process of restriction digests?
- What is the application of performing restriction digests?