Title: Demonstrate how manipulation of nucleic acids through genetic engineering alters the function of proteins and subsequent cellular processes.
Course: Biotechnology Foundations in Technology Education (21999) > Section: Investigating Genetic Engineering

Demonstration may include the following techniques of DNA extraction, manipulation, and analysis:

- Centrifugation
- Precipitation
- Spooling
- Electrophoresis
- Restriction enzymes
- Karyotypes
- Polymerase chain reaction (PCR)

Process/Skill Questions:

- What laboratory equipment is used for the extraction of DNA?
- What lab safety precautions should be taken when performing DNA extraction?
- What part do centrifugation, precipitation, and spooling play in extraction of DNA?
- What is PCR?