Performance should include

• using PPE (e.g., gloves, lab coat, eye protection)
• following universal precautions and all standard veterinary laboratory and sanitation protocols
• selecting appropriate flotation solutions (e.g., simulated sodium nitrate, simulated zinc sulfate)
• collecting, preparing, handling, labeling, and storing simulated fecal samples, using biohazard precautions (using simulants or homemade simulated stool)
• following established laboratory steps
  • mixing simulated fecal material with simulated flotation medium
  • straining to remove debris
  • filling flotation devices to form a positive meniscus
  • placing a coverslip for egg collection
• using a microscope with correct magnification and systematic scanning patterns to identify and document parasitic ova and debris
• following biohazard disposal protocols and disinfection of all surfaces according to OSHA and CDC guidelines.

Teacher Resources:

• Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University
• Collection and Submission of Laboratory Samples from Animals, Merck Veterinary Manual
• Module 2.3: Fecal Flotation, Clinical Veterinary Diagnostic Laboratory, University of Minnesota
• Resources, National Association of Veterinary Technicians in America (NAVTA)

Process/Skill Questions:

• What PPE is required when handling fecal samples?
• When should fecal flotation be performed on an animal?
• Why is flotation solution important in identifying parasite ova?
• How do you properly collect and store a fecal sample before testing?
• Where should fecal samples be disposed of after analysis?