Title: Perform a fecal flotation procedure using simulants or commercially prepared fecal flotation kits.
Course: Veterinary Science II (18105-II) > Section: Understanding Laboratory Procedures

Performance should include

- using PPE (e.g., gloves, lab coat, eye protection)
- following universal precautions and all standard veterinary laboratory and sanitation protocols
- selecting appropriate flotation solutions (e.g., simulated sodium nitrate, simulated zinc sulfate)
- collecting, preparing, handling, labeling, and storing simulated fecal samples, using biohazard precautions (using simulants or homemade simulated stool)
- following established laboratory steps
  - mixing simulated fecal material with simulated flotation medium
  - straining to remove debris
  - filling flotation devices to form a positive meniscus
  - placing a coverslip for egg collection
- using a microscope with correct magnification and systematic scanning patterns to identify and document parasitic ova and debris
- following biohazard disposal protocols and disinfection of all surfaces according to OSHA and CDC guidelines.

Teacher Resources:

- Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University
- Collection and Submission of Laboratory Samples from Animals, Merck Veterinary Manual
- Module 2.3: Fecal Flotation, Clinical Veterinary Diagnostic Laboratory, University of Minnesota
- Resources, National Association of Veterinary Technicians in America (NAVTA)

Process/Skill Questions:

- What PPE is required when handling fecal samples?
- When should fecal flotation be performed on an animal?
- Why is flotation solution important in identifying parasite ova?
- How do you properly collect and store a fecal sample before testing?
- Where should fecal samples be disposed of after analysis?